Fluorescence and luminescence-based technologies are at the forefront of biochemical research and drug discovery, utilising light emission for sensitive, quantitative measurements of molecular interactions, enzymatic activities, and cellular responses.
At o2h, we specialise in fluorescence-based studies, offering enzyme activity assays and techniques such as FRET (Fluorescence Resonance Energy Transfer) to elucidate protein-protein interactions. These interactions are crucial for understanding cellular signalling pathways and disease mechanisms.
Our services also include fluorescence polarization and quenching assays to investigate ligand-receptor interactions, which are essential for drug screening, and fluorescent dyes/indicators to monitor intracellular changes, such as calcium flux and variations in membrane potential.
On the luminescence front, we assess ATP levels through luciferase enzymes, which serve as metrics for evaluating cell viability and metabolic status. Our luminescent reporter assays elucidate gene expression dynamics, providing insights into transcriptional regulation and therapeutic targets.
At o2h, we also leverage advanced luminescence-based technologies, including BRET (Bioluminescence Resonance Energy Transfer) and BiT (Bimolecular Luminescence Complementation) assays, to explore protein-protein interactions and cellular dynamics.
These techniques enable real-time monitoring of interactions within live cells, offering valuable insights into signalling pathways and molecular mechanisms, while allowing for quantitative assessments of biological processes.
Together, these capabilities enhance our drug discovery efforts and contribute to a deeper understanding of complex biological systems.
To know more about our biology services offering or to request our brochure, please reach out to us at discovery@o2h.com.
