At o2h discovery, we deliver biophysical characterization of ligand-target interactions and can aid in hit finding approaches using fragment-based screening. We can prove mechanistic insights, kinetic and thermodynamic profiling for your novel compounds. With a published crystal structure of your target of interest and in complex with its cognate peptide/molecule, we can develop customizable assays for small molecule screening.
We can design Fluorescence Polarization (or) Anisotropy with labelled peptide or compound probes using high performance plate readers like CLARIOstar and PHERAstar. With label-free technology platforms like Biacore T200, we can establish more advanced studies like Surface Plasmon Resonance (SPR) providing vital kinetic data on ligand-target interaction like association-Kon, dissociation-Koff rates and KD determination. We can use simple techniques such as Thermal Shift Assay (TSA) to determine thermal unfolding and aggregation of the target protein using SYPRO Orange dye that binds to hydrophobic regions of the denatured proteins; illuminating the presence of unstable proteins in the absence of compound binding or target engagement.
In addition to the range of aforementioned biophysical methods, o2h discovery has well-established alliance partners to augment our capabilities and in providing tailored needs for your research project.
To know more about our biology services offering or to request our brochure, please reach out to us at discovery@o2h.com.
