The expression levels of genes within a cell population are in a state of flux dependent on a number of different extracellular or intracellular stimuli. As a result of this, the modulation of a target protein using a small molecule can often result in the expression of related genes also being affected. This allows us to use gene expression to assess the activity profile of a compound and ascertain whether it’s target engagement (assessed using our suite of biochemical & biophysical assays), translates to downstream transcriptional effects within a cellular environment.
At o2h Discovery, we can study changes in gene expression using quantitative RT-PCR to detect changes at the mRNA level and develop luciferase gene reporter assays to address pathway specific transcriptional activity.
We also offer bespoke assays and can generate suitable luciferase reporter cell lines, multiplex gene expression studies using suitable fluorescent probes and study alternative gene splicing across different species.
Our StepOnePlus Applied Biosystems and CFX96 Bio-Rad are 96-well Real time PCR instruments that can be setup in a variety of configurations with temperature control, and utilizes LED based multiple colour optical recording, suitable for multiplexing and can be utilized for a variety of genomic applications.
To know more about our biology services offering or to request our brochure, please reach out to us at discovery@o2h.com.