Mechanism of Action (MoA) studies play a pivotal role in drug discovery by clarifying how a drug exerts its effects at various biological levels. Understanding the MoA not only informs drug design but also helps predict potential side effects and guides clinical development. By investigating how drug molecules interact with specific cellular targets, researchers can provide critical insights into the impact of small molecules on biological systems. This understanding is vital for optimising dosing strategies and minimising harmful side effects, thereby reducing the risk of drug failure during clinical trials.
At o2h, we specialise in studies that broaden the understanding of molecular targets and the downstream signalling pathways responsive to candidate drug molecules. Our comprehensive approach focuses on target-specific inhibition, profiling compounds to minimize off-target activities.
Key components of our MoA studies:
- Target Identification and Profiling
- We identify molecular targets, including membrane receptors, ion channels, enzymes, and transcription factors, and assess the activation of downstream signalling pathways using various assays
- Customised Assay Development
- Our team develops and validates tailored assays employing techniques such as high-content imaging, gene expression analysis, and studies of post-translational modifications (e.g., phosphorylation) through methods like Western blotting and flow cytometry.
- Gain-of-Function and Loss-of-Function Studies
- We utilise gene expression modulation techniques, including RNA interference (siRNA, shRNA), overexpression, and CRISPR methodologies (knock-out, knock-in), and site-directed mutagenesis to investigate protein levels, target activity, and modifications.
- Protein and Biomolecule Interaction Bioassays
- Through assays such as co-immunoprecipitation (Co-IP), pull-down assays, bioluminescence resonance energy transfer (BRET), and fluorescence resonance energy transfer (FRET), we evaluate the affinity, specificity, kinetics, and stoichiometry of drug-target interactions.
By integrating these methodologies, o2h ensures a comprehensive investigation of drug mechanisms, ultimately contributing to the successful development of effective and safe therapeutic agents.
To know more about our biology services offering or to request our brochure, please reach out to us at discovery@o2h.com.
