SDM is a technique, which enables introducing desired targeted point mutations in the gene of interest. Of the many applications, the technique is of special importance to study the structure and function of protein. SDM can introduce a nucleotide substitution, deletion or addition. It is even possible to introduce deletion or insertion of a short stretch of DNA. The technique relies on the principle of providing a template sequence with the intended change in the primers. Using a pair of primers carrying the desired mutation to be introduced in the double stranded DNA, it is possible to alter the protein coding sequence as per the requirement. It is also possible to efficiently introduce multi-site mutagenesis. o2h offers a broad range of SDM services which includes a single or multi-site point mutations, insertions as well as deletions.
In the absence of crystal structure, various mutants and their wildtype can be characterized for their biophysical binding aiding in SAR development and lead optimization. Through collaborative approaches using structural and computational information, we can assist in understanding the binding mode of lead molecules using site-directed mutagenesis.
To know more about our biology services offering or to request our brochure, please reach out to us at discovery@o2h.com.
